Journal: The Journal of Biological Chemistry
Article Title: Nrf2b, Novel Zebrafish Paralog of Oxidant-responsive Transcription Factor NF-E2-related Factor 2 (NRF2) *
doi: 10.1074/jbc.M111.260125
Figure Lengend Snippet: Altered gene expression in embryos in which Nrf2a or Nrf2b has been knocked down by injection with Nrf2a-MO or Nrf2b-MO in comparison with those injected with a control-MO. Embryos were sampled at 52 hpf for analysis of gene expression by microarray or QPCR. A, Venn diagrams showing the numbers of probes up-regulated or down-regulated in each group of morphants, as compared with controls. B, expression of p53, ccng1, and hmox1 in Nrf2-morphant or control-MO-injected embryos. Data presented are the mean ± S.E. (error bars), where n = 5–6 pools of five embryos from two independent experiments. Non-injected and control-MO groups were not statistically different and were combined for graphical simplicity. *, statistical significance of Nrf2b-MO from all other groups (p < 0.05).
Article Snippet: The RNA samples ( n = 3 biological replicates per treatment/MO combination) were labeled with Cy3 and hybridized to the Agilent V3 4 × 44K zebrafish microarray (catalog no. G2519F-026437) at the Genome Technology Core of the Whitehead Institute (Cambridge, MA) using methods described in detail previously ( 34 ).
Techniques: Expressing, Injection, Microarray